Classification and analyses of hits obtained in the primary and secondary siRNA screens. (A) Schematic illustrating annotated functions of hits identified in the primary genome-wide siRNA screen. Functions were retrieved from the universal protein resource database (UniProt). Note that one gene can have multiple annotated functions; therefore, numbers here do not match those provided in the supplemental tables. (B) Graph depicting hits that were unique to STx2 in the primary screen and predicted to localize to endosomes/Golgi. (C) Secondary screen data. Each gene was targeted with four separate siRNAs.