Figure 1. asc is expressed during zebrafish early development. asc whole-mount, in situ hybridization (wish) of 3-dpf zebrafish larvae (A) with cross (B) and longitudinal (C) sectioning of plastic embedded wish sample showing expression in epidermis, intestinal epithelium, and cells located in the brain. Bars: (full larvae) 300 µm; (sections) 100 µm. Immunostaining of ASC in 3-dpf Tg(krt4:GFP) larva (D). Optical cross section of lateral fin showing GFP expression in the EVL and ASC expression on both epidermal layers (D′). WT 3-dpf larva immunostained for ASC, together with nuclear envelope marker lamin and DAPI shows its nuclear and cytoplasmic localization (E). Immunostaining of 3-dpf Tg(mpeg1:EGFP) (F), Tg(lyz:DsRed2) (G), and Tg(spi1b:GAL4,UAS:TagRFP) (H) larvae showing expression of ASC in macrophages, neutrophils, and a single myeloid cell in the caudal hematopoietic tissue (H, white arrowhead). Bars: (full larvae) 300 µm; (all others) 30 µm.
Figure 1.

asc is expressed during zebrafish early development. asc whole-mount, in situ hybridization (wish) of 3-dpf zebrafish larvae (A) with cross (B) and longitudinal (C) sectioning of plastic embedded wish sample showing expression in epidermis, intestinal epithelium, and cells located in the brain. Bars: (full larvae) 300 µm; (sections) 100 µm. Immunostaining of ASC in 3-dpf Tg(krt4:GFP) larva (D). Optical cross section of lateral fin showing GFP expression in the EVL and ASC expression on both epidermal layers (D′). WT 3-dpf larva immunostained for ASC, together with nuclear envelope marker lamin and DAPI shows its nuclear and cytoplasmic localization (E). Immunostaining of 3-dpf Tg(mpeg1:EGFP) (F), Tg(lyz:DsRed2) (G), and Tg(spi1b:GAL4,UAS:TagRFP) (H) larvae showing expression of ASC in macrophages, neutrophils, and a single myeloid cell in the caudal hematopoietic tissue (H, white arrowhead). Bars: (full larvae) 300 µm; (all others) 30 µm.

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