Figure 3. Ultrastructural phenotyping of... | Rockefeller University Press

Figure 3.

$Figure 3. Ultrastructural phenotyping of yeast with different genetic backgrounds subjected to osmotic shock. (A) Overlay of pseudocolor composite fluorescent barcode image and medium-magnification EM map of one grid square of the sample represents how alignment is performed between the LM and EM. The region in the white rectangle is shown in detail in Fig. S2, A–D. (B) Representative MVB cross sections from the laboratory strain S90, wine isolate PRICVV50, and BY4741 with a Δhog1 mutation. (C) MVB volume fractions in total cell volume in different strains and conditions; error bars show SEM calculated as described in Materials and methods. (D) MVB outer membrane surface-to-volume ratio in different strains and conditions; error bars show SEM. In C and D, 510 MVBs in 1,748 cell cross sections were analyzed. Scale bars: 20 µm in A, 100 nm in B.$

Ultrastructural phenotyping of yeast with different genetic backgrounds subjected to osmotic shock. (A) Overlay of pseudocolor composite fluorescent barcode image and medium-magnification EM map of one grid square of the sample represents how alignment is performed between the LM and EM. The region in the white rectangle is shown in detail in Fig. S2, A–D. (B) Representative MVB cross sections from the laboratory strain S90, wine isolate PRICVV50, and BY4741 with a Δhog1 mutation. (C) MVB volume fractions in total cell volume in different strains and conditions; error bars show SEM calculated as described in Materials and methods. (D) MVB outer membrane surface-to-volume ratio in different strains and conditions; error bars show SEM. In C and D, 510 MVBs in 1,748 cell cross sections were analyzed. Scale bars: 20 µm in A, 100 nm in B.