Figure 5. Impact of Fin1 phosphomutants on kinetochore stoichiometry. (a) The localization of Fin1-GFP in anaphase is shown. The only source of Fin1 is the copy on a CEN plasmid. Fin1 is expressed from its endogenous promoter. Bar, 5 µm. (b) The four strains in c have similar growth profiles. (c) The normalized fluorescence intensity measurements and anaphase/G1 ratios for Nuf2-GFP are shown. *P, <0.0001. Error bars indicate SEM. (d) The normalized fluorescence intensity measurements and anaphase/G1 ratios for Dsn1-GFP (MIND) in the WT and Fin1-5A background are shown. *P, <0.0001. Error bars indicate SEM. (e) The normalized fluorescence intensity measurements and anaphase/G1 ratios for Ask1-GFP (Dam1) in the WT and Fin1-5A background are shown. Error bars indicate SEM.
Figure 5.

Impact of Fin1 phosphomutants on kinetochore stoichiometry. (a) The localization of Fin1-GFP in anaphase is shown. The only source of Fin1 is the copy on a CEN plasmid. Fin1 is expressed from its endogenous promoter. Bar, 5 µm. (b) The four strains in c have similar growth profiles. (c) The normalized fluorescence intensity measurements and anaphase/G1 ratios for Nuf2-GFP are shown. *P, <0.0001. Error bars indicate SEM. (d) The normalized fluorescence intensity measurements and anaphase/G1 ratios for Dsn1-GFP (MIND) in the WT and Fin1-5A background are shown. *P, <0.0001. Error bars indicate SEM. (e) The normalized fluorescence intensity measurements and anaphase/G1 ratios for Ask1-GFP (Dam1) in the WT and Fin1-5A background are shown. Error bars indicate SEM.

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