Figure 2. Ultrastructural characterization of the kinetochore shows Fin1 colocalization with outer components in anaphase. (a) SPA-SIM of kinetochore proteins in metaphase (top, SPB distance <2 µm) and anaphase (bottom, SPB distance >2 µm) aligned to either Fin1 (where present) or Spc42. The GFP-Fin1 sample is aligned to Spc42, while the Fin1-GFP sample is aligned to Fin1. Bar, 200 nm; and all images are averages of >24 individual images. (b and c) 75% contour maps of the data from a. (d and e) Distance plots for centers of Gaussian fits to the distributions in panel a.
Figure 2.

Ultrastructural characterization of the kinetochore shows Fin1 colocalization with outer components in anaphase. (a) SPA-SIM of kinetochore proteins in metaphase (top, SPB distance <2 µm) and anaphase (bottom, SPB distance >2 µm) aligned to either Fin1 (where present) or Spc42. The GFP-Fin1 sample is aligned to Spc42, while the Fin1-GFP sample is aligned to Fin1. Bar, 200 nm; and all images are averages of >24 individual images. (b and c) 75% contour maps of the data from a. (d and e) Distance plots for centers of Gaussian fits to the distributions in panel a.

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