Figure 2. Mitochondrial detachment is uncoupled from the meiotic divisions and spore development. (A) Schematic of meiotic chromosome segregation and spore development. Meiotic regulators and spore development genes are labeled at key stages for their functions, where disruption of their function perturbs meiotic progression. (B–H) Maximum-intensity projections of fixed wild-type and mutant cells at 0 h (top) and 8 h (bottom) in SPO. Mitochondria (mito), mitoGFP or Cit1-GFP; nuclei, Htb1-mCherry. Dashed lines: cell boundaries. (B) Wild type (UB7155). (C) cdc28-as1 synchronized by pGAL-NDT80 GAL4.ER (UB9494), with 1 µM 1-NM-PP1 and 1 µM β-estradiol added simultaneously at 5 h. (D) cdc5-mn, which is pCLB2-CDC5 (UB7278). (E) cdc20-mn, which is pCLB2-CDC20 (UB7343). (F) spo12Δ (UB7345). (G) ama1Δ (UB7533). (H) spo21Δ synchronized by pGAL-NDT80 GAL4.ER (UB9239). Scale bar, 2 µm.
Figure 2.

Mitochondrial detachment is uncoupled from the meiotic divisions and spore development. (A) Schematic of meiotic chromosome segregation and spore development. Meiotic regulators and spore development genes are labeled at key stages for their functions, where disruption of their function perturbs meiotic progression. (B–H) Maximum-intensity projections of fixed wild-type and mutant cells at 0 h (top) and 8 h (bottom) in SPO. Mitochondria (mito), mitoGFP or Cit1-GFP; nuclei, Htb1-mCherry. Dashed lines: cell boundaries. (B) Wild type (UB7155). (C) cdc28-as1 synchronized by pGAL-NDT80 GAL4.ER (UB9494), with 1 µM 1-NM-PP1 and 1 µM β-estradiol added simultaneously at 5 h. (D) cdc5-mn, which is pCLB2-CDC5 (UB7278). (E) cdc20-mn, which is pCLB2-CDC20 (UB7343). (F) spo12Δ (UB7345). (G) ama1Δ (UB7533). (H) spo21Δ synchronized by pGAL-NDT80 GAL4.ER (UB9239). Scale bar, 2 µm.

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