Figure 3. Kinesin-1 recruitment to MTs depends on MAP7 family proteins. (A and B) Widefield images of K560-GFP overexpressed in the indicated HeLa control, knockout (KO), or knockdown (KD) conditions (A) and quantification of K560-GFP localization (B). n = 234, 252, 174, and 254 cells from three independent experiments. (C and D) Widefield images of K560-GFP overexpressed either alone or together with mCherry-tagged MAP7 constructs in control or MAP7 KO + siMAP7D1/D3 HeLa cells, as indicated. In C, cells were costained for α-tubulin. A schematic drawing of K560-GFP localization is shown at the bottom. (E and F) Quantification of K560-GFP (E) and MAP7 construct (F) localization per condition, as indicated, categorized as diffuse, along MTs, or at corner MTs. n = 459 (WT + siLuciferase), n = 485 (MAP7 KO + siMAP7D1/D3), and for rescue conditions, n = 113, 237, 90, 167, 210, 186, 133, 176, 197, and 193 cells from two to four independent experiments.
Figure 3.

Kinesin-1 recruitment to MTs depends on MAP7 family proteins. (A and B) Widefield images of K560-GFP overexpressed in the indicated HeLa control, knockout (KO), or knockdown (KD) conditions (A) and quantification of K560-GFP localization (B). n = 234, 252, 174, and 254 cells from three independent experiments. (C and D) Widefield images of K560-GFP overexpressed either alone or together with mCherry-tagged MAP7 constructs in control or MAP7 KO + siMAP7D1/D3 HeLa cells, as indicated. In C, cells were costained for α-tubulin. A schematic drawing of K560-GFP localization is shown at the bottom. (E and F) Quantification of K560-GFP (E) and MAP7 construct (F) localization per condition, as indicated, categorized as diffuse, along MTs, or at corner MTs. n = 459 (WT + siLuciferase), n = 485 (MAP7 KO + siMAP7D1/D3), and for rescue conditions, n = 113, 237, 90, 167, 210, 186, 133, 176, 197, and 193 cells from two to four independent experiments.

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