Figure 2. Characterization of MT-binding domains of MAP7. (A) Scheme of MAP7 truncations. MT binding was assayed by overexpression of GFP-tagged constructs and costain for α-tubulin; examples are shown in C. FL, full length; MTBD, MT binding domain. (B) MT pelleting assay with mCherry-MAP7-Ct analyzed by SDS-PAGE. Uncropped gel images are shown in Fig. S1 G. (C and D) Indicated GFP-tagged MAP7 constructs were overexpressed in MAP7 knockout HeLa cells costained for α-tubulin (C) to quantify their MT enrichment by line-scan analysis (D). n = 10 cells representing 30 MTs (3 per cell) per condition.
Figure 2.

Characterization of MT-binding domains of MAP7. (A) Scheme of MAP7 truncations. MT binding was assayed by overexpression of GFP-tagged constructs and costain for α-tubulin; examples are shown in C. FL, full length; MTBD, MT binding domain. (B) MT pelleting assay with mCherry-MAP7-Ct analyzed by SDS-PAGE. Uncropped gel images are shown in Fig. S1 G. (C and D) Indicated GFP-tagged MAP7 constructs were overexpressed in MAP7 knockout HeLa cells costained for α-tubulin (C) to quantify their MT enrichment by line-scan analysis (D). n = 10 cells representing 30 MTs (3 per cell) per condition.

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