CoQ domains are spatially linked to ER–mitochondria contact sites in cells. (A) Single-plane images of yeast cells expressing Coq9-mCherry (red), Ltc1-yEGFP (green), and mito-TagBFP (blue). Arrows indicate regions analyzed by line scan of fluorescence intensity indicated by dashed lines. Y axis is fluorescence intensity (AU), and x axis is distance (µm). (B) Single-plane superresolution images of yeast cells expressing Coq9-yEGFP (green), ER-DsRed (ER, red), and mito-TagBFP (mitochondria, blue). Arrows indicate regions analyzed by line scan of fluorescence intensity indicated by dashed lines. Y axis is fluorescence intensity (AU), and x axis is distance (µm). Scale bars = 2 µm.