Figure 5. Reduction in aster centering stiffness drives aster decentration and asymmetric division. (A) Time-lapse of a 3D simulation with constant pulling cap and aster centering stiffness reduction. The red hemisphere is the cap, MTs are in green and the aster trajectory in blue. (B) Examples of comparisons between simulated (top) and experimental (bottom) aster trajectories. (C) Aster position relative to the cell center in 3D simulations using positions of caps and sperm entry from experiments (in blue; n = 18). Blue dashed lines: SD. The red dashed line: the experimental curve (shown in Fig. 2 B). (D and E) Comparison of aster distances from the cell center and aster speeds in simulations (blue) and experiments (red). Error bars are SEM. (F) Schematized consecutive aster centration and decentration and model for asymmetric division driven by a reduction in aster MT centering stiffness under constant cortical forces.
Figure 5.

Reduction in aster centering stiffness drives aster decentration and asymmetric division. (A) Time-lapse of a 3D simulation with constant pulling cap and aster centering stiffness reduction. The red hemisphere is the cap, MTs are in green and the aster trajectory in blue. (B) Examples of comparisons between simulated (top) and experimental (bottom) aster trajectories. (C) Aster position relative to the cell center in 3D simulations using positions of caps and sperm entry from experiments (in blue; n = 18). Blue dashed lines: SD. The red dashed line: the experimental curve (shown in Fig. 2 B). (D and E) Comparison of aster distances from the cell center and aster speeds in simulations (blue) and experiments (red). Error bars are SEM. (F) Schematized consecutive aster centration and decentration and model for asymmetric division driven by a reduction in aster MT centering stiffness under constant cortical forces.

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