Dam1C/DASH rings can be visualized in cryosections. (A) Dam1C/DASH rings and MTs were assembled in vitro, high-pressure frozen, and then cryosectioned. Top row, cryotomographic slices (6 nm) of Dam1C/DASH rings around MTs. Bottom row, dashed lines corresponding to Dam1C/DASH (green) and MT (magenta) densities have been superposed on a copy of the top panel. (B) Some distorted Dam1C/DASH rings have portions that appear on different planes.