Figure 6.
Figure 6. APPL1 and Dyn1 regulate β1 integrin recycling. (A) Endocytosis of biotinylated β1 integrin scFv (5 µg/ml) or mAb K20 IgG (1 µg/ml) in H1975 cells. Percentage of internalized antibody was calculated relative to the initial surface bound. (B) Endocytosis of biotinylated β1 integrin scFv (5 µg/ml) was measured in control (Ctrl), Dyn1 cKO, and APPL1 cKO H1975 cells. Shown is the percentage of internalized scFv after 10 min at 37°C. (C) Recycling of biotinylated β1 integrin scFv (5 µg/ml) in control, Dyn1 cKO, and APPL1 cKO H1975 cells. Percentage of recycled β1 integrin scFv was calculated relative to the initial loading (10 min). (D) Surface levels of β1 integrin in Dyn1 and APPL1 cKO cells normalized to control. Error bars represent SEM (n ≥ 4 independent experiments). Unpaired t tests were used to assess statistical significance; **, P < 0.01; ***, P < 0.001.

APPL1 and Dyn1 regulate β1 integrin recycling. (A) Endocytosis of biotinylated β1 integrin scFv (5 µg/ml) or mAb K20 IgG (1 µg/ml) in H1975 cells. Percentage of internalized antibody was calculated relative to the initial surface bound. (B) Endocytosis of biotinylated β1 integrin scFv (5 µg/ml) was measured in control (Ctrl), Dyn1 cKO, and APPL1 cKO H1975 cells. Shown is the percentage of internalized scFv after 10 min at 37°C. (C) Recycling of biotinylated β1 integrin scFv (5 µg/ml) in control, Dyn1 cKO, and APPL1 cKO H1975 cells. Percentage of recycled β1 integrin scFv was calculated relative to the initial loading (10 min). (D) Surface levels of β1 integrin in Dyn1 and APPL1 cKO cells normalized to control. Error bars represent SEM (n ≥ 4 independent experiments). Unpaired t tests were used to assess statistical significance; **, P < 0.01; ***, P < 0.001.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal