Figure 4.
Figure 4. Zas1 binds the DNA sequence motif CCCCAY. (A) Sequence alignment of the Zas1-UAS motifs identified within the promoter sequences with highest ChIP-seq peaks. Numbers indicate base pairs relative to the transcription start site. (B) Positions of the Zas1-UAS 5′-CCCCAY-3′ (right red arrow) and its reverse complement sequence (left red arrow) of genes controlled by Zas1. (C) Zas1 ChIP-seq enrichment scores of peak regions with (red) or without (gray) Zas1-UAS sequences. (D) EMSA of recombinantly purified full-length Zas1 protein and 39-bp dsDNA substrates that contain unmodified (red) or mutated (white) Zas1-UAS motifs from the cnd1 or pob1 promoter sequences. Numbers above the lanes indicate the molar ratio of Zas1 protein to DNA. SDS-PAGE shows the purified Zas1 protein after staining with Coomassie blue.

Zas1 binds the DNA sequence motif CCCCAY. (A) Sequence alignment of the Zas1-UAS motifs identified within the promoter sequences with highest ChIP-seq peaks. Numbers indicate base pairs relative to the transcription start site. (B) Positions of the Zas1-UAS 5′-CCCCAY-3′ (right red arrow) and its reverse complement sequence (left red arrow) of genes controlled by Zas1. (C) Zas1 ChIP-seq enrichment scores of peak regions with (red) or without (gray) Zas1-UAS sequences. (D) EMSA of recombinantly purified full-length Zas1 protein and 39-bp dsDNA substrates that contain unmodified (red) or mutated (white) Zas1-UAS motifs from the cnd1 or pob1 promoter sequences. Numbers above the lanes indicate the molar ratio of Zas1 protein to DNA. SDS-PAGE shows the purified Zas1 protein after staining with Coomassie blue.

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