Genome-wide mapping of Zas1-binding sites. (A) Genomic positions of the 60 highest-scoring Zas1-binding sites identified by ChIP-seq. (B) Enrichment score plot for 3,017 sites in the fission yeast genome identified by Zas1-PK₆ ChIP-seq. Essential gene names are shown in dark blue, nonessential gene names in yellow, and ncRNA in gray. (C) ChIP-seq profiles from two independent experiments of a strain expressing Zas1-PK₆ (C4120) or an untagged control strain (C28) at the top five binding loci (SPBC887.16, SPC1652.02, SPBC713.13/SPBC713.14c, puc1, and peg1) and the condensin subunit gene cnd1. The y-axis indicates the number of reads per 20-bp bin. (D) Relative mRNA levels in zas1⁺ wild-type and zas1-Δaj3 mutant strains (obtained from tetrad dissection of a zas1-Δaj3/zas1⁺ strain, C4718) measured by RT-qPCR (mean ± SD; n = 8 replicates from four independent tetrads).