Figure 5.
Figure 5. Newly identified factors localize along the secretory pathway. (A–C) BioPlex 2.0 analysis displaying interacting partners of TTC17 (A), CCDC151 (B), and C10orf88 (C). Functional proteins acting along the secretory pathway or cargo proteins transported along the ER-Golgi apparatus are highlighted in red. (D–H) HeLa cells were processed for cytosolic washout. Then, the colocalization of CCDC151 (D and E), TTC17 (F), CCDC157 (G), and C10orf88 (H; green) with TGN46 or centrin 3 (red) was assessed by immunofluorescence microscopy. Nuclei were stained with DAPI. Scale bars, 20 µm in D and F–H. Scale bars, 8 µm in E. Intensity profile graphs showing the relative localization of the indicated proteins were obtained along the lines shown in the confocal micrograph inserts. (I) Pearson’s correlation coefficient of colocalization for the indicated proteins (r) derived from confocal micrographs (n = 30).

Newly identified factors localize along the secretory pathway. (A–C) BioPlex 2.0 analysis displaying interacting partners of TTC17 (A), CCDC151 (B), and C10orf88 (C). Functional proteins acting along the secretory pathway or cargo proteins transported along the ER-Golgi apparatus are highlighted in red. (D–H) HeLa cells were processed for cytosolic washout. Then, the colocalization of CCDC151 (D and E), TTC17 (F), CCDC157 (G), and C10orf88 (H; green) with TGN46 or centrin 3 (red) was assessed by immunofluorescence microscopy. Nuclei were stained with DAPI. Scale bars, 20 µm in D and F–H. Scale bars, 8 µm in E. Intensity profile graphs showing the relative localization of the indicated proteins were obtained along the lines shown in the confocal micrograph inserts. (I) Pearson’s correlation coefficient of colocalization for the indicated proteins (r) derived from confocal micrographs (n = 30).

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