Summarizing model. The structural and dynamic information gathered from our experiments define a comprehensive model of higher-order chromatin organization as follows: RD median size is 150 nm and ranges up to ∼400 nm. RDs comprise four co-replicating regions separated by 60 nm on average. The typical nearest-neighbor spacing (center to center) between RDs is significantly bigger than typical RD size, with a median of 300 nm and ranges up to ∼600 nm. We hypothesize the existence of extended linker regions between RDs with a median length of 150 nm. The elastic coupling range between RDs is lost at distances >550 nm.