Gating strategy for flow cytometry–based analysis of dermal and lung cells. Flow cytometry strategy for analyzing dermal and lung single-cell suspensions for the expression of GFP, the association of cells with fluorescent collectins, or the expression of uPARAP. GFP was expressed under the col1a1 promotor (using col1a1.GFP mice), whereas fluorescent collectins (MBL [dermal cells] and SP-D [lung cells]) and fluorescent anti-uPARAP antibody were taken up by cells in vivo. Single-cell suspensions were stained for CD45, F4/80, CD11b, EpCAM, and CD31. CD45⁻ cells (nonleukocytes) were separated into EpCAM⁺ epithelial cells, CD31⁺ endothelial cells, and EpCAM⁻CD31⁻ cells. Fibroblasts were identified as GFP⁺ in the EpCAM⁻CD31⁻ population. CD45⁺ cells (leukocytes) were divided into F4/80⁺CD11b⁺ (macrophages), F4/80⁻CD11b⁺ (nonmacrophage, myeloid cells), and CD11b⁻ (nonmyeloid leukocytes).