The formation of large protein aggregates depends on translation. (A–C) Localization patterns of mCherry-VHL (A), Hsp104-GFP (B), and Hsp42 (C) in S. cerevisiae cells and their superposition with the nuclear marker Htb1-cerulean (A and B) or DNA stained by DAPI (C) upon control conditions (30°C), and proteotoxic stress in the absence or presence of CHX (37°C for 30 min + MG132 ± CHX) are shown. Maximal projections of widefield z stack images with corresponding quantifications (n > 50/sample) are presented. Bars, 2 µm.