Figure 6. In vivo cross-linking of Brr6 and Brl1 recruits Nup188. (A) Principle of 3GBP in vivo cross-linking assay. (B) Growth of indicated cells with plasmids p415Gal or p415Gal-3GBP. (C and E) p415Gal or p415Gal-3GBP cells were incubated for 3 h at 30°C with galactose to induce the PGAL1 promoter. The enlargements show NEs that were used for the plot profiles on the right. Arrowheads indicate colocalization of Brr6/Brl1 and Nup188. Bars: (overviews) 5 µm; (enlargements) 1 µm. (D and F) Quantification of cells from C and E. Error bars: SD (n > 300 for C and n > 150 for E); three independent experiments. Unpaired t test with two-tailed p-value was used to compare the samples. ***, P ≤ 0.001; ****, P ≤ 0.0001; ns, not significant.
Figure 6.

In vivo cross-linking of Brr6 and Brl1 recruits Nup188. (A) Principle of 3GBP in vivo cross-linking assay. (B) Growth of indicated cells with plasmids p415Gal or p415Gal-3GBP. (C and E) p415Gal or p415Gal-3GBP cells were incubated for 3 h at 30°C with galactose to induce the PGAL1 promoter. The enlargements show NEs that were used for the plot profiles on the right. Arrowheads indicate colocalization of Brr6/Brl1 and Nup188. Bars: (overviews) 5 µm; (enlargements) 1 µm. (D and F) Quantification of cells from C and E. Error bars: SD (n > 300 for C and n > 150 for E); three independent experiments. Unpaired t test with two-tailed p-value was used to compare the samples. ***, P ≤ 0.001; ****, P ≤ 0.0001; ns, not significant.

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