Brr6 and Brl1 interact with structural NUPs that are required for the early NPC assembly. (A) BiFC fluorescent signals of cells expressing Brl1-VC and NUP-VN fusions. A cartoon of the NPC with indicated VN-fusions is shown. Bar, 5 µm. (B) Quantification of cells from A. Error bars: SD (n > 100); three independent experiments. (C) Ndc1-6HA coimmunoprecipitates with Brr6-3yeGFP. (D) Nup188-6HA and Ndc1-6HA copurifies with yeGFP-Brr6 and yeGFP-Brl1.