Figure 3. Overexpression of BRR6 or BRL1 does not cause lipid alterations, but BRL1 overexpression rescues growth of nup116Δ cells. (A) Growth of WT and nup116Δ cells with the indicated plasmids. (B–E) Lipid composition analysis of WT, PGAL1-BRR6 and PGAL1-BRL1 cells (6-h induction at 30°C) by nano-ESI-MS/MS. The description is the same as in Fig. 2 (B–E). Error bars in B–E: SD (n = 3). Unpaired t test with two-tailed p-value was used to compare the samples in D; *, P ≤ 0.05. (F) Growth of yeast cells on BA plates. (G) NPC phenotype of WT and brr6(ts) and brl1(ts) cells incubated for 3 h at 37°C. Arrowheads indicate GFP-dots in the cytoplasm. Bar, 5 µm.
Figure 3.

Overexpression of BRR6 or BRL1 does not cause lipid alterations, but BRL1 overexpression rescues growth of nup116Δ cells. (A) Growth of WT and nup116Δ cells with the indicated plasmids. (B–E) Lipid composition analysis of WT, PGAL1-BRR6 and PGAL1-BRL1 cells (6-h induction at 30°C) by nano-ESI-MS/MS. The description is the same as in Fig. 2 (B–E). Error bars in B–E: SD (n = 3). Unpaired t test with two-tailed p-value was used to compare the samples in D; *, P ≤ 0.05. (F) Growth of yeast cells on BA plates. (G) NPC phenotype of WT and brr6(ts) and brl1(ts) cells incubated for 3 h at 37°C. Arrowheads indicate GFP-dots in the cytoplasm. Bar, 5 µm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal