Figure 2. Codepletion of Brr6 and Brl1 does not affect lipid composition. (A) Cells were incubated at 37°C as in Fig. 1 B and analyzed 0, 3, and 6 h after temperature shift with Nup188-yeGFP as NPC marker. Images with maximum-intensity projections are shown. Bar, 5 µm. (B–F) Lipids were extracted from cell cultures used for A with subsequent mass spectrometric analysis by nano-ESI-MS/MS. Samples in B–E were analyzed after 3 h at 37°C. (B) Lipid classes displayed as mol% of measured lipids. (C) Distribution of lipids into functional categories of glycerophospholipids and glycerolipids (GPL), sphingolipids (SP), sterols (ST), and storage lipids. (D) Chain length profiles. (E) Double bond profiles. (F) Changes in the mol% distributions of lipid classes during the time course experiment. Error bars in B–F: SD (n = 3).
Figure 2.

Codepletion of Brr6 and Brl1 does not affect lipid composition. (A) Cells were incubated at 37°C as in Fig. 1 B and analyzed 0, 3, and 6 h after temperature shift with Nup188-yeGFP as NPC marker. Images with maximum-intensity projections are shown. Bar, 5 µm. (B–F) Lipids were extracted from cell cultures used for A with subsequent mass spectrometric analysis by nano-ESI-MS/MS. Samples in B–E were analyzed after 3 h at 37°C. (B) Lipid classes displayed as mol% of measured lipids. (C) Distribution of lipids into functional categories of glycerophospholipids and glycerolipids (GPL), sphingolipids (SP), sterols (ST), and storage lipids. (D) Chain length profiles. (E) Double bond profiles. (F) Changes in the mol% distributions of lipid classes during the time course experiment. Error bars in B–F: SD (n = 3).

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