Figure 4. AMOT-130 stabilizes the actin... | Rockefeller University Press

$Figure 4. AMOT-130 stabilizes the actin cytoskeleton in dendritic spines. (A) FRAP results of YFP–AMOT-130 and derivative AMOT constructs in neurons treated with DMSO or 10 nM jasplakinolide (Jasp) as indicated. Time-lapse images show spine turnover of the indicated constructs before and at 0, 9, 27, and 60 s after photobleaching. The pseudo color scale is indicated at the bottom. Bar, 2 µm. (B) Normalized data fit in single exponential decay curves of fluorescence recovery (y-axis) plotted against time after bleaching (x-axis). (C) Quantification of AMOT recovery in dendritic spines. (D) FRAP results of GFP-actin alone or expressed together with Flag–AMOT-130 or Flag–AMOT-130–ΔC. Time-lapse images show spine protein turnover before and at 0, 9, 27, and 60 s after photobleaching. Dashed circles indicate photobleached spines. (E) Normalized data fit in single exponential decay curves of fluorescence recovery (y-axis) plotted against time after bleaching (x-axis). (F) Quantification of GFP-actin recovery in dendritic spines. Error bars represent means ± SD of the mobile fraction after photobleaching. *, P < 0.05; **, P < 0.01; one-way ANOVA followed by Tukey’s post hoc test.$

Figure 4.

AMOT-130 stabilizes the actin cytoskeleton in dendritic spines. (A) FRAP results of YFP–AMOT-130 and derivative AMOT constructs in neurons treated with DMSO or 10 nM jasplakinolide (Jasp) as indicated. Time-lapse images show spine turnover of the indicated constructs before and at 0, 9, 27, and 60 s after photobleaching. The pseudo color scale is indicated at the bottom. Bar, 2 µm. (B) Normalized data fit in single exponential decay curves of fluorescence recovery (y-axis) plotted against time after bleaching (x-axis). (C) Quantification of AMOT recovery in dendritic spines. (D) FRAP results of GFP-actin alone or expressed together with Flag–AMOT-130 or Flag–AMOT-130–ΔC. Time-lapse images show spine protein turnover before and at 0, 9, 27, and 60 s after photobleaching. Dashed circles indicate photobleached spines. (E) Normalized data fit in single exponential decay curves of fluorescence recovery (y-axis) plotted against time after bleaching (x-axis). (F) Quantification of GFP-actin recovery in dendritic spines. Error bars represent means ± SD of the mobile fraction after photobleaching. *, P < 0.05; **, P < 0.01; one-way ANOVA followed by Tukey’s post hoc test.

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