Localization of the SPIN components upon NE insertion of the Spc42–Spc29 obelisk. (A) Fluorescence intensity quantification of the yeGFP signal of indicated proteins at the SPB after release from the G1 block in WT cells (shaded portions in graphs) and cells upon 45 min SPC42 SPC29 OE (scatter plot). Error bars indicate SD; n ≥ 50. The black horizontal line indicates the maximum yeGFP intensity at the duplicated SPB without SPC42 SPC29 OE. RFI, relative fluorescence intensity. (B) SIM images of SPC42 SPC29 OE cells in α-factor arrest and after 120 min release. Bars: (main images) 2 µm; (insets) 500 nm.