E-cadherin relaxation and β-catenin translocation are non–cell autonomous. (A) Top: Typical micrographs of GFP-β-catenin cells either single or doublet cells with or without HGF (50 ng/µL). Bottom: Normalized area of cells with or without HGF (data from Fig. S1 I) after 2 h and for single cells after 2 h of HGF treatment (n = 10 cells). (B) GFP intensity in the nucleus (relative to cytoplasm) of GFP-β-catenin 5 h with or without HGF (50 ng/ml; data from Fig. 1 E) and for single cells under HGF (n = 26 cells). (C) FRET index change at cell–cell contacts of EcadTSMod cells 5 h with and without HGF (50 ng/ml; data from Fig. 1 D), and for single cells at the basal membrane 5 h with HGF (n = 53 cells). (D) Typical micrographs of GFP-β-catenin cells with and without HGF (50 ng/ml) for island of cells at low density (LD) and confluent cells at high density (HD). (E) GFP intensity in the nucleus (relative to cytoplasm) of GFP-β-catenin 5 h with or without HGF (50 ng/ml) at LD (data from Fig. 1 E), and confluence (HD; n = −/+ 75/40 cells). (F) FRET index change at cell–cell contacts of EcadTSMod in cells 5 h with and without HGF (50 ng/ml) at LD (data from Fig. 1 D) and confluence (HD; n = +/− 27/30 cells). Bars, 20 µm. Two-tailed Kruskal–Wallis test. Values plotted are mean ± SEM.