E-cadherin tension relaxation correlates with β-catenin nuclear accumulation. (A) Wounded sheet of MDCK cells expressing EcadTSMod. Top: Direct YFP fluorescence. Middle: Corresponding FRET index map. Note that this map does not display protein levels. Bottom: FRET index in leader cells lamellipodia (n = 315 cells) and cell–cell contacts at the back (∼500 µm; n = 410) 10 h postwound. (B) GFP-β-catenin in a wounded MDCK sheet. Top: Typical map. Bottom: GFP intensity (relative to back cells) in leader cells (n = 32 cells) and cells at the back (n = 120; ∼500 µm) 10 h postwound. (C) TOPdGFP in a wounded MDCK sheet. Top: Typical map. Bottom: GFP intensity (relative to back cells) in leader cells (n = 20 cells) and cells at the back (n = 21; ∼500 µm) 10 h postwound. (D) FRET index of EcadTSMod in cells with and without HGF (50 ng/ml). Top: Typical maps. Bottom: FRET index change at cell–cell contacts 5 h after HGF addition (n = 72 cells) versus control (n = 173). (E) GFP-β-catenin in cells with and without HGF (50 ng/ml). Top: Typical maps. Bottom: GFP intensity in the nucleus (relative to cytoplasm) 5 h after HGF addition (n = 47 cells) versus control (n = 42). Bars: (wound healing) 100 µm; (HGF) 20 µm. Two-tailed Mann–Whitney test. Values plotted are mean ± SEM.