WT LMNA and LMNA(R482W) differentially associate with the MEST/MIR335 locus after adipogenic induction. (A) ChIP-qPCR analysis of LMNA in undifferentiated and differentiated ASC^EGFP, ASC^LMNAWT, and ASC^LMNA(R482W) (means ± SD of biological triplicates; ANOVA p-values: r1 Undiff., *, P = 0.026; r1 Diff., *, P = 0.003; r3 Diff., *, P = 0.001; ^nsP > 0.1). (B) Western blot assessment of expression of LMNA WT and R482W in ASCs. (C) FISH analysis of the MEST/MIR335 locus (arrows) in native ASCs, ASC^LMNAWT, and ASC^LMNA(R482W) before and after 3 d of adipogenic induction. DNA was stained with DAPI. Bars, 5 µm. (D) ChIP-qPCR analysis of LMNB1 in ASC^EGFP, ASC^LMNAWT, and ASC^LMNA(R482W) (^nsANOVA p-values ∼1.000; means ± SD of triplicate ChIPs).