Figure 9. Rab8a/Rab11a GAPs interact with Stx3. (A) TBC1D30 and Evi5 interact with Stx3 and partially with proteins of apical cargo trafficking in CaCo2 cells. Strep-Tactin was used for coaffinity purification (co-AP). (B) TBC1D30 and Stx3 interact directly in vitro. GST alone was used as a negative control. (C) Evi5 and Stx3 interact directly in vitro. GST alone was used as a negative control. The asterisk indicates the GST degradation background band. (D) In vitro interaction of Snap23a and Stx3 was used as a positive control for B and C. (E) Evi5 localizes at the apical plasma membrane in WT but appears dispersed in the cytoplasm upon deletion of Stx3 (gStx3). Arrowheads on the left mark the corresponding XY and XZ planes. Bars, 10 µm. IB, immunoblotting.
Figure 9.

Rab8a/Rab11a GAPs interact with Stx3. (A) TBC1D30 and Evi5 interact with Stx3 and partially with proteins of apical cargo trafficking in CaCo2 cells. Strep-Tactin was used for coaffinity purification (co-AP). (B) TBC1D30 and Stx3 interact directly in vitro. GST alone was used as a negative control. (C) Evi5 and Stx3 interact directly in vitro. GST alone was used as a negative control. The asterisk indicates the GST degradation background band. (D) In vitro interaction of Snap23a and Stx3 was used as a positive control for B and C. (E) Evi5 localizes at the apical plasma membrane in WT but appears dispersed in the cytoplasm upon deletion of Stx3 (gStx3). Arrowheads on the left mark the corresponding XY and XZ planes. Bars, 10 µm. IB, immunoblotting.

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