Deletion of Myo5B and Stx3, but not Vamp3, results in selective cargo mislocalization. (A) Apical localization of SI seems unaltered upon deletion of Myo5B or Stx3 (gSTX3). (B) Apical localization of DPPIV seems unaltered upon deletion of Vamp3 (gVamp3). (C) Apical localization of HS-NHE3 seems unaltered upon deletion of Vamp3 (gVamp3). Arrowheads on the left mark the corresponding XY and XZ planes. Quantification and statistical analysis of indicated cargo/enzyme localization is shown to the right of corresponding confocal micrographs. Mean ± SD. ***, P < 0.005. n ≥ 100 cells. Bars, 10 µm. api, apical/brush border plane; mid, middle/nuclear plane.