Myo5B mediates selective cargo transport. (A) NHE3 resides apically in WT (brush border actin is stained), but mislocalizes throughout the cytoplasm upon Myo5B deletion. Bars, 10 µm. (B) GLUT5 localizes at the apical plasma membrane in WT, but appears dispersed in the cytoplasm upon deletion of Myo5B. DPPIVs apical localization seems unaffected. Arrowheads on the left mark the corresponding XY and XZ planes. Quantification and statistical analysis of indicated cargo/enzyme localization is shown to the right of corresponding confocal micrographs. Bars, 10 µm. Mean ± SD. ***, P < 0.005. n ≥ 100 cells. api, apical/brush border plane; mid, middle/nuclear plane.