Figure 3. Stx3, Rab8a, and Rab11a are pivotal for epithelial polarity. (A) Rab8a_Q67L partly colocalizes with Stx3 at the AMIS (third and fourth columns). (B) Localization of Rab11a_Q70L changes from the periphery to the AMIS, colocalizing with Stx3 as soon as lumen formation starts (third and fourth columns). (C) Myo5B localizes to the periphery (first and second columns) but shows high colocalization with Stx3 at the AMIS (third and fourth columns) when lumen formation begins. (D) Depletion of Stx3 (gSTX3), Rab8a (gRab8a), and Rab11a (shRab11a) results in disrupted cyst polarity (7 d). The apical enzyme DPPIV still localizes correctly (green). (E) Quantification of single lumen formation. Mean ± SD. ***, P < 0.005. n ≥ 100 cysts/experiment; three independent experiments. White boxes in A–C indicate detailed views in separate colors. Bars: (A–C) 10 µm; (magnifications) 1 µm; (D) 10 µm.
Figure 3.

Stx3, Rab8a, and Rab11a are pivotal for epithelial polarity. (A) Rab8a_Q67L partly colocalizes with Stx3 at the AMIS (third and fourth columns). (B) Localization of Rab11a_Q70L changes from the periphery to the AMIS, colocalizing with Stx3 as soon as lumen formation starts (third and fourth columns). (C) Myo5B localizes to the periphery (first and second columns) but shows high colocalization with Stx3 at the AMIS (third and fourth columns) when lumen formation begins. (D) Depletion of Stx3 (gSTX3), Rab8a (gRab8a), and Rab11a (shRab11a) results in disrupted cyst polarity (7 d). The apical enzyme DPPIV still localizes correctly (green). (E) Quantification of single lumen formation. Mean ± SD. ***, P < 0.005. n ≥ 100 cysts/experiment; three independent experiments. White boxes in A–C indicate detailed views in separate colors. Bars: (A–C) 10 µm; (magnifications) 1 µm; (D) 10 µm.

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