Figure 2. Analysis of the Rab11/Rab8 cascade in CaCo2. (A) Rab8a_Q67L interacts with proteins of the apical trafficking route: Sec15, Slp4a, Stx3, Rab11a, and Rab3b. Strep-Tactin was used for coaffinity purification (co-AP). (B) Same as is in A with Rab11a_Q70L as bait and Rab8a interaction. (C) Myo5B interacts with all proteins of the apical trafficking route (compare with A and B). (D) Stx3 localizes correctly during cyst development of Myo5B KI cells despite the overall disrupted polarity (left to right: 24 h, 48 h, 3 d, and 7 d); AMIS is marked by white arrowheads. Bars, 10 µm.
Figure 2.

Analysis of the Rab11/Rab8 cascade in CaCo2. (A) Rab8a_Q67L interacts with proteins of the apical trafficking route: Sec15, Slp4a, Stx3, Rab11a, and Rab3b. Strep-Tactin was used for coaffinity purification (co-AP). (B) Same as is in A with Rab11a_Q70L as bait and Rab8a interaction. (C) Myo5B interacts with all proteins of the apical trafficking route (compare with A and B). (D) Stx3 localizes correctly during cyst development of Myo5B KI cells despite the overall disrupted polarity (left to right: 24 h, 48 h, 3 d, and 7 d); AMIS is marked by white arrowheads. Bars, 10 µm.

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