Figure 4. Accumulation of mouse HA-Dicer in damaged nuclei. (A) Confocal imaging (top) detecting endogenously tagged, mouse HA-Dicer in primary MEF cell line PMEF::HA-Dicer. Control, MEF wild type. Quantitation as the percentage of cells with nuclear HA signal. n > 50 (bottom left). *, P < 0.05; error bars, means ± SEM of three biological replicates and immunoblot detecting HA-Dicer (bottom right). WCE, whole cell extract; Ponceau S staining, loading control. (B) Confocal imaging of heterokaryon fusions between human AsiSI-ER U2OS cells and mouse PMEF::HA-Dicer or wild-type MEF cells, respectively. Quantitations are shown as signal sums (mean intensity × area). Nuclei: H.s., Homo sapiens, full circle; M.m., Mus musculus, dashed circle. Number of analyzed fused cells n > 15.
Figure 4.

Accumulation of mouse HA-Dicer in damaged nuclei. (A) Confocal imaging (top) detecting endogenously tagged, mouse HA-Dicer in primary MEF cell line PMEF::HA-Dicer. Control, MEF wild type. Quantitation as the percentage of cells with nuclear HA signal. n > 50 (bottom left). *, P < 0.05; error bars, means ± SEM of three biological replicates and immunoblot detecting HA-Dicer (bottom right). WCE, whole cell extract; Ponceau S staining, loading control. (B) Confocal imaging of heterokaryon fusions between human AsiSI-ER U2OS cells and mouse PMEF::HA-Dicer or wild-type MEF cells, respectively. Quantitations are shown as signal sums (mean intensity × area). Nuclei: H.s., Homo sapiens, full circle; M.m., Mus musculus, dashed circle. Number of analyzed fused cells n > 15.

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