Figure 3. Nuclear accumulation of S1016 phosphorylated Dicer upon DNA damage in HEK293 cells. (A) Schematic of human Dicer isoform 1 (NP_001258211.1) domain structure and positions of assessed phosphorylated residues. DUF283, domain of unknown function; PAZ, Piwi/Argonaute/Zwille; RIIIa/b, RNase III a/b; dsRBD, double-stranded RNA binding domain. (B) Confocal imaging of RFP-tagged Dicer constructs expressed in wild-type HEK293 cells. All quantifications represent the number of cells that have the shown phenotype. (C) Relative quantification of (B). Bars, mean ratio (nuclear/cytoplasmic RFP) normalized to the background, n > 45. (D) Absolute quantification of (B). Bars, mean number of cells with nuclear RFP signal, n > 45. *, P < 0.05.
Figure 3.

Nuclear accumulation of S1016 phosphorylated Dicer upon DNA damage in HEK293 cells. (A) Schematic of human Dicer isoform 1 (NP_001258211.1) domain structure and positions of assessed phosphorylated residues. DUF283, domain of unknown function; PAZ, Piwi/Argonaute/Zwille; RIIIa/b, RNase III a/b; dsRBD, double-stranded RNA binding domain. (B) Confocal imaging of RFP-tagged Dicer constructs expressed in wild-type HEK293 cells. All quantifications represent the number of cells that have the shown phenotype. (C) Relative quantification of (B). Bars, mean ratio (nuclear/cytoplasmic RFP) normalized to the background, n > 45. (D) Absolute quantification of (B). Bars, mean number of cells with nuclear RFP signal, n > 45. *, P < 0.05.

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