Figure 9. Rab11aS25N expression perturbs polycystin-2 trafficking to the primary cilium. (A) Selected images of NRK cells expressing Flag-Rab11a, Flag-Rab11aS25N, or Flag-Rab11aQ70L stained for polycystin-2 (Pkd2) antibody (red), acetylated tubulin (6-11B-1) antibody (green), and Flag antibody (blue). Bars, 10 µm. Insets are 200% enlargements of the cilia. (B) Quantification of mean total ciliary polycystin-2 levels. Ciliary polycystin-2 is reduced in Flag-Rab11aS25N-expressing cells. (C) Quantification of mean total ciliary polycystin-2/cilia length. Ciliary polycystin-2 per cilium length is reduced in Flag-Rab11aS25N-expressing cells. (D) Quantification of mean cilia length. There is no difference in ciliary assembly when the Rab11a constructs are expressed. n = 85 cilia per experimental group. Error bars are SEM. Data were analyzed using one-way ANOVA and the Bonferroni multiple-comparisons test. ****, P < 0.0001.
Figure 9.

Rab11aS25N expression perturbs polycystin-2 trafficking to the primary cilium. (A) Selected images of NRK cells expressing Flag-Rab11a, Flag-Rab11aS25N, or Flag-Rab11aQ70L stained for polycystin-2 (Pkd2) antibody (red), acetylated tubulin (6-11B-1) antibody (green), and Flag antibody (blue). Bars, 10 µm. Insets are 200% enlargements of the cilia. (B) Quantification of mean total ciliary polycystin-2 levels. Ciliary polycystin-2 is reduced in Flag-Rab11aS25N-expressing cells. (C) Quantification of mean total ciliary polycystin-2/cilia length. Ciliary polycystin-2 per cilium length is reduced in Flag-Rab11aS25N-expressing cells. (D) Quantification of mean cilia length. There is no difference in ciliary assembly when the Rab11a constructs are expressed. n = 85 cilia per experimental group. Error bars are SEM. Data were analyzed using one-way ANOVA and the Bonferroni multiple-comparisons test. ****, P < 0.0001.

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