Figure 7. Ciliary levels of polycystin-2 but not smoothened are reduced in GFP-MyoVb-overexpressing cells. (A) NRK cells expressing GFP-MyoVb C-terminal tail. (Aa) Polycystin-2 (Pkd2) antibody staining (red), Arl13b antibody staining (green), and GFP-MyoVb (blue). Polycystin-2 and Arl13b colocalize with GFP-MyoVb at the recycling endosome (arrows). Bars, 10 µm. Insets are 150% enlargements of the cilia. (Ab) Mean steady-state ciliary polycystin-2 is decreased, (Ac) ciliary polycystin-2/cilia is reduced, and (Ad) there is no difference in mean cilia length in NRK cells expressing GFP-MyoVb. (B) NIH 3T3 Ift27−/− cells expressing GFP-MyoVb C-terminal tail. (Ba) Smoothened (Smo) antibody staining (red), GFP-MyoVb (green), and IFT88 antibody staining (blue). Modest levels of smoothened colocalizes with GFP-MyoVb at the recycling endosome (arrows). Bar, 10 µm. Insets are 150% enlargements of the cilia. (Bb) No difference in mean steady-state ciliary smoothened, (Bc) total ciliary smoothened/length is decreased, and (Bd) there is no difference in mean ciliary length in GFP-MyoVb-expressing cells. n = 102 cilia per experimental group. Error bars are SEM. Data were analyzed using the unpaired Student’s t test. *, P < 0.05; **, P < 0.01; ****, P < 0.0001.
Figure 7.

Ciliary levels of polycystin-2 but not smoothened are reduced in GFP-MyoVb-overexpressing cells. (A) NRK cells expressing GFP-MyoVb C-terminal tail. (Aa) Polycystin-2 (Pkd2) antibody staining (red), Arl13b antibody staining (green), and GFP-MyoVb (blue). Polycystin-2 and Arl13b colocalize with GFP-MyoVb at the recycling endosome (arrows). Bars, 10 µm. Insets are 150% enlargements of the cilia. (Ab) Mean steady-state ciliary polycystin-2 is decreased, (Ac) ciliary polycystin-2/cilia is reduced, and (Ad) there is no difference in mean cilia length in NRK cells expressing GFP-MyoVb. (B) NIH 3T3 Ift27−/− cells expressing GFP-MyoVb C-terminal tail. (Ba) Smoothened (Smo) antibody staining (red), GFP-MyoVb (green), and IFT88 antibody staining (blue). Modest levels of smoothened colocalizes with GFP-MyoVb at the recycling endosome (arrows). Bar, 10 µm. Insets are 150% enlargements of the cilia. (Bb) No difference in mean steady-state ciliary smoothened, (Bc) total ciliary smoothened/length is decreased, and (Bd) there is no difference in mean ciliary length in GFP-MyoVb-expressing cells. n = 102 cilia per experimental group. Error bars are SEM. Data were analyzed using the unpaired Student’s t test. *, P < 0.05; **, P < 0.01; ****, P < 0.0001.

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