Blocking phosphorylation on T275 and S278 of Sid4 to block Chk2^Cds1 recruitment abolishes Chk2^Cds1’s ability of to evict Flp1.GFP from the SPB. (A and B) Representative fluorescence and brightfield (BF) images of flp1.GFP ppc89.tdTom cells that reveal how the eviction of Flp1.GFP from the SPB is abolished in the sid4.T275AS278A background that prevents Chk2^Cds1 recruitment to the SPB. (C) Quantification of the data in A and B. n = 3. For controls, see Fig. S4. (D) Frequency of monopolarity in the indicated strains as for Fig. 1 B. Error bars in C and D indicate SD for three independent experiments.