Figure 5. SYX-5 controls MVB fusion with the apical plasma membrane. (A) In the epidermis, SYX-5 localizes in both large cytoplasmic puncta and smaller apical puncta. (B) SYX-5 displays some colocalization with VHA-5 in the epidermis at the time of alae formation. (C–E) SYX-5 colocalizes with RAL-1(CA) (C), but not with RAL-1(DN) (D), as revealed by quantification (E). The scale bars in B apply to C and D. The numbers inside the bars indicate the number of puncta (number of animals). (F) Generation of two mutant alleles for syx-5 using CRISPR/Cas9 technology. (G) Homozygote mutants for syx-5(mc51) show alae defects at the L1 stage. (H) Electron micrographs of two MVBs showing attachment to the apical plasma membrane in syx-5(mc51) mutant larva. (I) Model for the role of RAL-1 and SYX-5 in exosome biogenesis (see text). Errors bars, SEM.
Figure 5.

SYX-5 controls MVB fusion with the apical plasma membrane. (A) In the epidermis, SYX-5 localizes in both large cytoplasmic puncta and smaller apical puncta. (B) SYX-5 displays some colocalization with VHA-5 in the epidermis at the time of alae formation. (C–E) SYX-5 colocalizes with RAL-1(CA) (C), but not with RAL-1(DN) (D), as revealed by quantification (E). The scale bars in B apply to C and D. The numbers inside the bars indicate the number of puncta (number of animals). (F) Generation of two mutant alleles for syx-5 using CRISPR/Cas9 technology. (G) Homozygote mutants for syx-5(mc51) show alae defects at the L1 stage. (H) Electron micrographs of two MVBs showing attachment to the apical plasma membrane in syx-5(mc51) mutant larva. (I) Model for the role of RAL-1 and SYX-5 in exosome biogenesis (see text). Errors bars, SEM.

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