The SNAREs Syx13, Snap29 and Vamp7 are required for crinophagy. Loss of Syx13, Snap29, and Vamp7 results in a glue granule degradation defect (A–D). Salivary gland-specific knockdown of Syx13 (B), Snap29 (C), or Vamp7 (D) inhibits the quenching of GFP within glue granules compared with control cells (A) at 0 h RPF. Glue granules fail to properly acquire lysosomal CathB in the absence of these SNAREs (E–H). Numerous Glue-GFP granules overlap with CathB-3xmCherry in control cells (E, arrowheads), which decreases upon silencing of Syx13 (F), Snap29 (G), or Vamp7 (H) in −2-h gland cells. Impaired glue granule fusion with late endosomes and lysosomes in SNARE RNAi cells (I–L). Formation of GFP-Lamp1 rings (arrowheads) around Glue-Red granules that is seen in control cells (I) is impaired in cells undergoing Syx13 (J), Snap29 (K), or Vamp7 (L) RNAi, indicating a fusion defect. Green and/or magenta channels are shown separately as indicated. Bars: (A–D) 20 µm; (E–L) 3 µm. Please see Fig. S3 (A–C) for quantification of data.