Mgr1 and Mgr3 interact with MOM substrates and facilitate substrate recruitment to Yme1. (A–F) Digitonin-solubilized mitochondrial extracts from the indicated WT and mutant strains were subject to anti-FLAG or anti-HA IP and analyzed by SDS-PAGE. 5 µl (A, B, and D–F) or 20 µl (C) out of 100 µl immunoprecipitates were loaded for the anti-HA (A, B, and D–F) or anti-Yme1 (C) blots. (G and H) Digitonin-solubilized mitochondrial extracts (10 µg) from the indicated WT and mutant strains were analyzed by BN-PAGE and SDS-PAGE. The boxed regions highlight high MW upshifts of Mgr1-FLAG and Mgr3-FLAG. Molecular masses are shown in kilodaltons.