Figure 7. Loop-axis model showing the... | Rockefeller University Press

Figure 7.

$Figure 7. Loop-axis model showing the dynamic behavior of Spp1 upon tethering. A subset of Spp1 binding sites (red hexagons) becomes tethered to the chromosome axis (ORFs in red). These tethered sites correspond to the dynamic fraction of Spp1 peaks identified by time resolved ChIP-Seq. Appearing Spp1 sites have the ability to interact with Mer2 and to tether DSB sites to the axis where they undergo Spo11-mediated DNA cleavage (yellow star). These properties depend on the PHD domain and the Mer2 binding motif of Spp1, as well as H3K4 and H3R2 methylation. Constant Spp1 sites (green hexagons) remain unchanged during the meiotic time course. Constant Spp1 sites do not interact with Mer2 and remain colocalized with Set1C (ORFs in green). Disappearing Spp1 sites (blue hexagons) are mainly associated with RPG and SnoRNA genes (ORFs in blue). Spp1 tends to be released from the Set1 holocomplex at the latter sites, reflecting the plasticity of Set1C.$

Loop-axis model showing the dynamic behavior of Spp1 upon tethering. A subset of Spp1 binding sites (red hexagons) becomes tethered to the chromosome axis (ORFs in red). These tethered sites correspond to the dynamic fraction of Spp1 peaks identified by time resolved ChIP-Seq. Appearing Spp1 sites have the ability to interact with Mer2 and to tether DSB sites to the axis where they undergo Spo11-mediated DNA cleavage (yellow star). These properties depend on the PHD domain and the Mer2 binding motif of Spp1, as well as H3K4 and H3R2 methylation. Constant Spp1 sites (green hexagons) remain unchanged during the meiotic time course. Constant Spp1 sites do not interact with Mer2 and remain colocalized with Set1C (ORFs in green). Disappearing Spp1 sites (blue hexagons) are mainly associated with RPG and SnoRNA genes (ORFs in blue). Spp1 tends to be released from the Set1 holocomplex at the latter sites, reflecting the plasticity of Set1C.