Micrographs of individual budding yeast cells identified during segmentation, with illustrative examples of four types of features that could be identified during feature extraction. In these micrographs, red pixels mark the cellular cytosol, whereas green pixels represent GFP-fusion proteins that localize to unique subcellular structures in each cell. Area features are concerned with the number of pixels in the segmented region, GFP intensity features consider overall green pixel brightness, shape features examine the contours of the cell objects, and texture examines the spatial arrangement of pixel intensities. These features, and many others, are quantified for each cellular object and then used in downstream clustering or classification.