Figure 4. Diffusion of single SunTagx56-Ki67 polysomes and mRNAs. (A) Legend as in Fig. 3 A, except that cells were imaged at 4 frames/s (fps) for 2 min. Bar, 10 µm. (B–D) Quantification of the diffusion rates of SunTagx56-Ki67 polysomes. In B, the graph is a histogram of 1D displacements measured between two consecutive video frames (n = 957). In C, the graph represents the mean MSD as a function of time, for the same 957 particles. In D, the histogram represents the diffusion coefficient of individual polysomes, measured for tracks longer than 36 s (n = 75). (E) Quantification of the diffusion rates of SunTagx56-Ki67 mRNAs. Legend as in Fig. 2 B, except that the SunTagx56-Ki67 mRNA were tracked using nls-MCP-TagRFPt (n = 2,021). Only cells with a majority of untranslated SunTagx56-Ki67 mRNA were analyzed.
Figure 4.

Diffusion of single SunTagx56-Ki67 polysomes and mRNAs. (A) Legend as in Fig. 3 A, except that cells were imaged at 4 frames/s (fps) for 2 min. Bar, 10 µm. (B–D) Quantification of the diffusion rates of SunTagx56-Ki67 polysomes. In B, the graph is a histogram of 1D displacements measured between two consecutive video frames (n = 957). In C, the graph represents the mean MSD as a function of time, for the same 957 particles. In D, the histogram represents the diffusion coefficient of individual polysomes, measured for tracks longer than 36 s (n = 75). (E) Quantification of the diffusion rates of SunTagx56-Ki67 mRNAs. Legend as in Fig. 2 B, except that the SunTagx56-Ki67 mRNA were tracked using nls-MCP-TagRFPt (n = 2,021). Only cells with a majority of untranslated SunTagx56-Ki67 mRNA were analyzed.

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