Treatment with low doses of latrunculin A prevents ring repair after laser microsurgery. (A and B) Schematics illustrating the procedure for embryo permeabilization (A) and live imaging combined with acute drug treatment (B). (C) Stills of control and latrunculin A–treated permeable embryos. (D) Stills of a latrunculin A–treated ABa cell, whose constricting ring was laser cut at the indicated time (cyan arrow). Time is in seconds. Bars, 5 µm.