Analysis of repair after laser microsurgery in contractile rings. (A) Kymograph of the cut ring region showing that ring curvature is recovered by the end of gap repair. (B) Time between the laser cut and completion of gap repair plotted against the initial perimeter (n = 38). (C) The perimeter at the time of completion of repair plotted against the initial perimeter, showing a strong correlation (n = 38; R² = 0.99 and r = 0.99; P < 0.0001). (D) Schematic illustrating how heat maps of myosin^NMY-2::GFP distribution along the contractile ring circumference over time shown in E were generated. (E) Heat maps for myosin^NMY-2::GFP as described in D. (F) Stills of two cut rings that fail the first repair attempt. Red arrows point to foci of myosin^NMY-2::GFP that accumulate de novo along the length of the gap. Time is in seconds, and time zero corresponds to the frame before the laser cut. Bars, 5 µm.