Figure 3. The diffusion of ER membrane protein becomes anisotropic between PNM and NEBD. (A) The timing of anisotropic diffusion onset for GFP::SP12 (n = 57) and RAMP4::YFP (n = 62) relative to the cell cycle stages PNM, NEBD, and anaphase. GFP::SP12; div-1(RNAi) (n = 35) shows longer cell cycle length, yet the anisotropy onset occurs in the similar time window. The blue shading indicates the PNM stage, the orange shading represents NEBD, and the red shading represents anaphase. Black error bars indicate the mean ± SD of the time of anisotropy onset; gray error bars indicate the mean ± SD of the time of PNM, NEBD, and anaphase for the RAMP::YFP and GFP::SP12 embryos; and green error bars indicate the mean ± SD for GFP::SP12; Ctrl(RNAi) (n = 34). (B) Box plots of the ratios of the time from anisotropic onset to NEBD over the time from PNM to NEBD for GFP::SP12 (n = 42), RAMP4::YFP (n = 45), GFP::SP12; div-1(RNAi) (n = 30), and Ctrl(RNAi) (n = 31). Whiskers indicate the minimum and maximum values. Unpaired t test, P < 0.05. ns, not significant. (C) Deconvolved images of a single plane in the middle (red) and closer to the cortex (green) of the GFP::SP12 embryo stained for GFP show the continuity of the ER membrane along the A-P axis (double-arrowed dashed line). Also see Video 7. Bars, 10 µm.
Figure 3.

The diffusion of ER membrane protein becomes anisotropic between PNM and NEBD. (A) The timing of anisotropic diffusion onset for GFP::SP12 (n = 57) and RAMP4::YFP (n = 62) relative to the cell cycle stages PNM, NEBD, and anaphase. GFP::SP12; div-1(RNAi) (n = 35) shows longer cell cycle length, yet the anisotropy onset occurs in the similar time window. The blue shading indicates the PNM stage, the orange shading represents NEBD, and the red shading represents anaphase. Black error bars indicate the mean ± SD of the time of anisotropy onset; gray error bars indicate the mean ± SD of the time of PNM, NEBD, and anaphase for the RAMP::YFP and GFP::SP12 embryos; and green error bars indicate the mean ± SD for GFP::SP12; Ctrl(RNAi) (n = 34). (B) Box plots of the ratios of the time from anisotropic onset to NEBD over the time from PNM to NEBD for GFP::SP12 (n = 42), RAMP4::YFP (n = 45), GFP::SP12; div-1(RNAi) (n = 30), and Ctrl(RNAi) (n = 31). Whiskers indicate the minimum and maximum values. Unpaired t test, P < 0.05. ns, not significant. (C) Deconvolved images of a single plane in the middle (red) and closer to the cortex (green) of the GFP::SP12 embryo stained for GFP show the continuity of the ER membrane along the A-P axis (double-arrowed dashed line). Also see Video 7. Bars, 10 µm.

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