Figure 6. Cross talk between endothelial and epithelial cells induces HGF in Mgp−/− lungs. (a and b) Epithelial cells (a) and ECs (b) isolated from Mgp+/+ and Mgp−/− lungs were treated with BMP-4, anti-VEGF or anti–BMP-4 (a-BMP-4) antibodies, or transfected with Flt1 siRNA. Expression of VEGF, Flt1, HGF, and c-Met was determined by real-time PCR after 24 h of treatment (n = 3). (c) Expression of albumin (Alb) in epithelial cells was isolated from Mgp+/+ and Mgp−/− lungs and treated with HGF for 24 h (n = 3). (d–f) Expression of VEGF, Flt1, HGF, and c-Met in ECs was isolated from Mgp+/+ and Mgp−/− lungs and individually treated with BMP-2, -7, and -9 (n = 3). (g) Schematic diagram of cross talk between ECs and epithelial cells. Data in a–f were analyzed by two-sided t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Error bars are standard deviation. Data distribution was assumed to be normal, but this was not formally tested.
Figure 6.

Cross talk between endothelial and epithelial cells induces HGF in Mgp−/− lungs. (a and b) Epithelial cells (a) and ECs (b) isolated from Mgp+/+ and Mgp−/− lungs were treated with BMP-4, anti-VEGF or anti–BMP-4 (a-BMP-4) antibodies, or transfected with Flt1 siRNA. Expression of VEGF, Flt1, HGF, and c-Met was determined by real-time PCR after 24 h of treatment (n = 3). (c) Expression of albumin (Alb) in epithelial cells was isolated from Mgp+/+ and Mgp−/− lungs and treated with HGF for 24 h (n = 3). (d–f) Expression of VEGF, Flt1, HGF, and c-Met in ECs was isolated from Mgp+/+ and Mgp−/− lungs and individually treated with BMP-2, -7, and -9 (n = 3). (g) Schematic diagram of cross talk between ECs and epithelial cells. Data in a–f were analyzed by two-sided t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Error bars are standard deviation. Data distribution was assumed to be normal, but this was not formally tested.

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