Decrease of HGF in ECs reduces hepatic differentiation in Mgp^−/− lungs. (a) Schematic diagram of breeding strategy to create Chd5^CreHgf^flox/floxMgp^−/− mice. (b) Genes involved in liver metabolism, with extraction of significant difference in expression (P < 0.05) derived from the gene expression profiles from lungs and liver of Cdh5^CreHgf^wt/wtMgp^+/+, Cdh5^CreHgf^flox/flox Mgp^+/+, Cdh5^CreHgf^wt/wt Mgp^−/−, and Cdh5^CreHgf^flox/flox Mgp^−/− mice (n = 2). (c) Expression of albumin (Alb), Ttr, Pah, and AFP was determined in lungs by real-time PCR (n = 3). (d) The total amount of albumin (right) and activity of P450 (left) was determined in cells isolated from lungs (n = 8). Data in c and d were analyzed by two-sided t test. ***, P < 0.001. Error bars are standard deviation. Data distribution was assumed to be normal, but this was not formally tested.