Depletion of MZT1 in C. albicans delocalizes Caγ-TuSC from SPBs and disrupts MT organization. (A) CaMzt1-GFP associates with SPBs throughout the cell cycle. Δ/Tetp-CaMZT1-GFP cells were grown with and without Dox to control CaMZT1-GFP expression. DNA was stained with DAPI. Bar, 10 µm. (B) The Caγ-TuSC component CaSpc97-GFP was delocalized from SPBs after repression of CaMZT1 by Dox. Δ/Tetp-CaMZT1 CaSPC97-GFP/CaSPC97 cells were grown with and without Dox. Bar, 10 µm. (C) Time-lapse of Δ/Tetp-CaMZT1 CaTUB1-GFP cells with and without Dox. Time zero was set as the budding onset. See Fig. S1 F for earlier time points of cells 1 and 2. Bars, 5 µm. Enlargements of boxed areas in the time-course experiment are shown (bars, 2 µm). The arrows mark the free MTs, and the asterisks mark the neck-associated MT arrays. (D) Repression of CaMZT1 expression led to the failure of spindle formation. Analysis of cells from C. (E) Duration of spindle assembly was estimated as interval between onset of budding and the start of spindle elongation (from C). Error bars are SEM. ***, P < 0.001. Ana/Telo, anaphase/telophase; M, metaphase.