Figure 4. Loss of SNAP23 results in decreased amylase secretion in parotid exocrine cells. (A) Phase-contrast image of cultured parotid exocrine cells at 3 and 48 h after Ad-LacZ and Ad-Cre infection. Bar, 50 µm. (B) Relative expression levels of SNAP23 mRNA measured by real-time PCR at 48 h after adenovirus infection. Parotid exocrine cells from two mice were examined. (C) SNAP23 and GP2 (ZG marker) staining in parotid exocrine cells. SNAP23 localized to the plasma membrane in the Ad-LacZ–infected exocrine cells, but the staining disappeared in the Ad-Cre–infected exocrine cells. Bar, 10 µm. (D) Amylase secretion is reduced in Ad-Cre infected exocrine cells. Infected exocrine cells were incubated with IPR and MeCh, and the amount of secreted amylase was measured. Parotid exocrine cells from three mice were examined. Data are mean ± SEM. Significance was calculated by the two-tailed paired Student’s t test. ***, P < 0.001.
Figure 4.

Loss of SNAP23 results in decreased amylase secretion in parotid exocrine cells. (A) Phase-contrast image of cultured parotid exocrine cells at 3 and 48 h after Ad-LacZ and Ad-Cre infection. Bar, 50 µm. (B) Relative expression levels of SNAP23 mRNA measured by real-time PCR at 48 h after adenovirus infection. Parotid exocrine cells from two mice were examined. (C) SNAP23 and GP2 (ZG marker) staining in parotid exocrine cells. SNAP23 localized to the plasma membrane in the Ad-LacZ–infected exocrine cells, but the staining disappeared in the Ad-Cre–infected exocrine cells. Bar, 10 µm. (D) Amylase secretion is reduced in Ad-Cre infected exocrine cells. Infected exocrine cells were incubated with IPR and MeCh, and the amount of secreted amylase was measured. Parotid exocrine cells from three mice were examined. Data are mean ± SEM. Significance was calculated by the two-tailed paired Student’s t test. ***, P < 0.001.

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